Serious Sam on Game Boy Advance is a first-person shooter where players control Sam as he travels back to ancient Rome through a timelock. Tasked with battling Mental's minions, Sam faces off against ten unique enemies and utilizes ten weapons throughout twelve action-packed levels. The game features elements familiar to fans of the Serious Sam franchise while adapting them for handheld play.
Scientists figure out how to use the timelock to send someone to any point in time, Sam travels back to ancient Rome as people haven't been returning from that period and ends up fighting Mental's minions once again.
The game has ten weapons and ten enemies, most of which are taken from the other Serious Sam games. It spans twelve levels.