G.I. Joe – The Rise of Cobra for PlayStation Portable follows Duke and Scarlett as they embark on a mission to rescue Heavy Duty, Ripcord, and Snake Eyes from a detention facility. The team combats the villainous Baroness and confronts Cobra's plan in Egypt involving the Apep gem. Ultimately, they face Destro and Cobra Commander in a climactic battle at a volcanic base.
After the events of the film, Duke and Scarlett travels to the Arctic and free Heavy Duty, Ripcord and Snake Eyes from the M.A.R.S Detention Center. Also, they fight the Baroness and bring her to the Pit. In Egypt, the Joes learn that Cobra is taking the Apep (Gem), and must defeat Firefly. In the jungle, the Joes learn that Cobra is making a pyramid-like building in the middle of the jungle. From there, the Joes must defeat James McCullen (who now takes the identity of Destro) at the rooftop of the pyramid building. Finally, they must defeat the Cobra Commander and Storm Shadow at North Magnetic Pole Volcano base.